The transient receptor potential channel TRPV6 is dynamically expressed in bone cells but is not crucial for bone mineralization in mice

32Citations
Citations of this article
30Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Bone is the major store for Ca 2+ in the body and plays an important role in Ca 2+ homeostasis. During bone formation and resorption Ca 2+ must be transported to and from bone by osteoblasts and osteoclasts, respectively. However, little is known about the Ca 2+ transport machinery in these bone cells. In this study, we examined the epithelial Ca 2+ channel TRPV6 in bone. TRPV6 mRNA is expressed in human and mouse osteoblast-like cells as well as in peripheral blood mononuclear cell-derived human osteoclasts and murine tibial bone marrow-derived osteoclasts. Also other transcellular Ca 2+ transport genes, calbindin-D 9k and/or -D 28K, Na +/Ca 2+ exchanger 1, and plasma membrane Ca 2+ ATPase (PMCA1b) were expressed in these bone cell types. Immunofluorescence and confocal microscopy on human osteoblasts and osteoclasts and mouse osteoclasts revealed TRPV6 protein at the apical domain and PMCA1b at the osteoidal domain of osteoblasts, whereas in osteoclasts TRPV6 was predominantly found at the bone-facing site. TRPV6 was dynamically expressed in human osteoblasts, showing maximal expression during mineralization of the extracellular matrix. 1,25-Dihydroxyvitamin D 3 (1,25(OH) 2D 3) did not change TRPV6 expression in both mineralizing and non-mineralizing SV-HFO cultures. Lentiviral transduction-mediated overexpression of TRPV6 in these cells did not alter mineralization. Bone microarchitecture and mineralization were unaffected in Trpv6 D541A/D541A mice in which aspartate 541 in the pore region was replaced with alanine to render TRPV6 channels non-functional. In summary, TRPV6 and other proteins involved in transcellular Ca 2+ transport are dynamically expressed in bone cells, while TRPV6 appears not crucial for bone metabolism and matrix mineralization in mice. © 2011 Wiley Periodicals, Inc.

Cite

CITATION STYLE

APA

Van der Eerden, B. C. J., Weissgerber, P., Fratzl-Zelman, N., Olausson, J., Hoenderop, J. G. J., Schreuders-Koedam, M., … Van Leeuwen, J. P. T. M. (2012). The transient receptor potential channel TRPV6 is dynamically expressed in bone cells but is not crucial for bone mineralization in mice. Journal of Cellular Physiology, 227(5), 1951–1959. https://doi.org/10.1002/jcp.22923

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free