Treatment of dry eye by autologous serum application in Sjögren's syndrome

  • Tsubota K
  • Goto E
  • Fujita H
 et al. 
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Aim—To evaluate the eYcacy of autolo- gous serum application for the treatment of dry eye in Sjögren’s syndrome. Methods—The stability of essential com- ponents (EGF, vitamin A, and TGF-􏰒) in preserved serum were examined following preservation at 4°C and −20°C. In a primary clinical trial, 12 patients with Sjögren’s syndrome were treated with autologous serum (diluted to 20% with sterile saline) for 4 weeks, and vital stain- ing of the ocular surface was compared before and after treatment. The eVects of serum on mucin (MUC-1) expression were observed in cultured conjunctival epithelial cells in vitro. Results—EGF, vitamin A, and TGF-􏰒 were well preserved for up to 1 month in the refrigerator at 4°C and up to 3 months in the freezer at −20°C. Rose bengal and fluorescein scores improved significantly from the initial scores of 5.3 and 5.6 to 1.7 and 2.5 after 4 weeks, respectively. The additive eVect of human serum for cul- tured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface. Conclusion—Autologous serum applica- tion is a safe and eYcient way to provide essential components to the ocular surface in the treatment of dry eye associated with Sjögren’s syndrome.

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  • Kazuo Tsubota

  • Eiki Goto

  • Hiromi Fujita

  • Masafumi Ono

  • Hiroko Inoue

  • Ichiro Saito

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