Aim‚ÄîTo evaluate the eYcacy of autolo- gous serum application for the treatment of dry eye in SjoÃàgren‚Äôs syndrome. Methods‚ÄîThe stability of essential com- ponents (EGF, vitamin A, and TGF-Ùè∞í) in preserved serum were examined following preservation at 4¬∞C and ‚àí20¬∞C. In a primary clinical trial, 12 patients with SjoÃàgren‚Äôs syndrome were treated with autologous serum (diluted to 20% with sterile saline) for 4 weeks, and vital stain- ing of the ocular surface was compared before and after treatment. The eVects of serum on mucin (MUC-1) expression were observed in cultured conjunctival epithelial cells in vitro. Results‚ÄîEGF, vitamin A, and TGF-Ùè∞í were well preserved for up to 1 month in the refrigerator at 4¬∞C and up to 3 months in the freezer at ‚àí20¬∞C. Rose bengal and fluorescein scores improved significantly from the initial scores of 5.3 and 5.6 to 1.7 and 2.5 after 4 weeks, respectively. The additive eVect of human serum for cul- tured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface. Conclusion‚ÄîAutologous serum applica- tion is a safe and eYcient way to provide essential components to the ocular surface in the treatment of dry eye associated with SjoÃàgren‚Äôs syndrome.
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