Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined the coverage of amplification by analyzing a SNP linkage marker set that contained 2320 SNP markers spread across the genome at an average distance of 2.5 cM. We observed a concordance of >99.8% in genotyping results from genomic DNA and amplified DNA, strongly indicating the ability of both methods used to amplify genomic DNA in a highly representative manner. Furthermore, we were able to achieve a SNP call rate of >98% in both genomic and amplified DNA. The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples. © 2004 by Cold Spring Harbor Laboratory Press.
CITATION STYLE
Barker, D. L., Hansen, M. S. T., Faraqi, A. F., Giannola, D., Irsula, I. R., Lasken, R. S., … Lai, E. (2004). Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Research, 14(5), 901–907. https://doi.org/10.1101/gr.1949704
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