Ul -regulation of nitric oxide synthase by estradiol in human aortic endothelial cells

  • Hishikawa K
  • Nakaki T
  • Marumo T
 et al. 
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Abstract

We have examined the effects of sex hormones on calcinm-dependent NO production and protein levels of NO syn-thase in cultured human aortic endothelial cells, which were treated with various doses of 17~-estradiol and testosterone for 8-48 h. Treatment with 17~-estradiol enhanced calcium-depend-ent NO production, but testosterone had exerted no effect. West-ern blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17~-estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.

Author-supplied keywords

  • Atherosclerosis
  • Estrogen
  • Human endothelial cell
  • NO synthase
  • Nitric oxide
  • Testosterone

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Authors

  • Keiichi Hishikawa

  • Toshio Nakaki

  • Takeshi Marumo

  • Hiromichi Suzuki

  • Ryuichi Kato

  • Takao Sarutaa

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