Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification

  • Nagino K
  • Nomura O
  • Takii Y
 et al. 
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Abstract

We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.

Author-supplied keywords

  • Gene Expression Profiling/instrumentation/*methods
  • Microscopy, Electron, Scanning
  • Nucleic Acid Amplification Techniques/*methods
  • Nucleic Acid Hybridization/methods
  • Oligonucleotide Array Sequence Analysis/instrument
  • Plasminogen Activators/genetics
  • RNA, Messenger/genetics/metabolism
  • RNA/genetics/*metabolism
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction/me
  • Statistics, Nonparametric

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Authors

  • K Nagino

  • O Nomura

  • Y Takii

  • A Myomoto

  • M Ichikawa

  • F Nakamura

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