Urine sediment examination: A comparison of automated urinalysis systems and manual microscopy

  • Chien T
  • Kao J
  • Liu H
 et al. 
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Abstract

Background: Several automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-100 and Iris iQ200 with manual microscopy in urine sediment testing. Methods: Four hundred and thirty-six urine samples were collected. The urine sediments were examined by manual microscopy and these 2 automated urinalysis systems. Results: The within-run CVs for urine samples ranged from 3.4% to 22.3% for the iQ200, 1.6% to 24.2% for the UF-100 and 12.5% to 43.9% for manual microscopy. Between-run CVs on quality-control samples ranged from 6.1% to 32.4% for the iQ200 and 3.5% to 24.7% for the UF-100. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.935 to 0.968. However, for epithelial cells, the values measured by different systems were poorly correlated (r = 0.888-0.922). The Bland-Altman plot indicated a trend towards the automated cell count being greater than the manual microscopy as the epithelial cell count increased. Casts were difficultly differentiated by 2 automated systems. Conclusions: These 2 automated urinalysis systems demonstrated good concordance with each other in urine sediment examination. The automated process could be used as a screening procedure but some manual microscopy was still necessary. © 2007 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Automation
  • Image
  • Urine microscopy
  • Urine sediment

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Authors

  • Tzu I. Chien

  • Jau Tsuen Kao

  • Hui Lan Liu

  • Po Chang Lin

  • Jhih Sian Hong

  • Han Peng Hsieh

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