Visual Dna - Identification of Dna Sequence Variations by Bead Trapping

  • Ståhl P
  • Gantelius J
  • Natanaelsson C
 et al. 
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Abstract

In this paper we describe a method that uses the nearly covalent strength biotin–streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera.

Author-supplied keywords

  • Imaging Techniques
  • Magnetics
  • Medical Imaging
  • Microspheres
  • Oligonucleotide Array
  • Sequence Analysis
  • Signal Amplification Assay

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Authors

  • P L Ståhl

  • J Gantelius

  • C Natanaelsson

  • A Ahmadian

  • H Andersson-Svahn

  • J Lundeberg

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