In this paper we describe a method that uses the nearly covalent strength biotin-streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera. © 2007 Elsevier Inc. All rights reserved.
CITATION STYLE
Ståhl, P. L., Gantelius, J., Natanaelsson, C., Ahmadian, A., Andersson-Svahn, H., & Lundeberg, J. (2007). Visual DNA - Identification of DNA sequence variations by bead trapping. Genomics, 90(6), 741–745. https://doi.org/10.1016/j.ygeno.2007.07.014
Mendeley helps you to discover research relevant for your work.