In vitro study of human vascular endothelial cell function on materials with various surface roughness

  • Xu C
  • Yang F
  • Wang S
 et al. 
  • 3

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

In recent years, creating a biodegradable polymer scaffold with an endothelialized surface has become an attractive concept for replacement of small-diameter blood vessels. Toward this end, a better understanding of the interaction between endothelial cells and biodegradable polymer substrates is particularly important. Surface roughness of biomaterials is one of the important parameters that affect cell behavior. In this study, human vascular endothelial cells were cultured on electrospun and solvent-cast poly(L-lactic acid) substrates with different surface roughness. Cell responses were evaluated via both qualitative examinations of cell morphology changes as well as quantitative assessment of cell adhesion and proliferation rate on the different substrates. The results proved that endothelial cell function was enhanced on the smooth solvent-cast surface rather than on the rough electrospun surface of Poly(L-lactic acid). Together with our previous findings that electrospun substrates favor vascular smooth muscle cell behavior, it is possible to design a unique three-dimensional scaffold for application of tissue-engineered small-diameter vessel replacement by combining the fabrication technique of solvent casting and electrospinning. (C) 2004 Wiley Periodicals, Inc.

Author-supplied keywords

  • adhesion
  • biodegradable polymers
  • differentiation
  • electrospinning
  • endothelial cells
  • expression
  • extracellular-matrix
  • growth
  • human bone-cells
  • osteoblast-like cells
  • poly (l-lactic acid)
  • proliferation
  • solvent cast
  • surface roughness
  • titanium

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • C Y Xu

  • F Yang

  • S Wang

  • S Ramakrishna

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free