In vitro T-cell generation from adult, embryonic, and induced pluripotent stem cells: Many roads to one destination

  • Smith M
  • Webber B
  • Mohtashami M
 et al. 
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Abstract

T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self-renewing source of T cells that can be readily genetically modified. T-cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T-cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex. In this review, we discuss current methods for the in vitro derivation of T cells from murine and human HSPCs and hPSCs that use feeder-cell and feeder-cell-free systems. Furthermore, we explore their potential for adoption for use in T-cell-based therapies.

Author-supplied keywords

  • CD34-+-
  • Cell culture
  • Cord blood
  • Differentiation
  • Embryonic stem cells
  • Hematopoietic stem cells
  • Induced pluripotent stem cells
  • T cells

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Authors

  • celalettin ustunUniversity of Minnesota - Twin Cities

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  • Michelle J. Smith

  • Beau R. Webber

  • Mahmood Mohtashami

  • Heather E. Stefanski

  • Juan Carlos Zúñiga-Pflücker

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