Skip to content
Journal article

In vivo gene delivery by cationic tetraamino fullerene.

Maeda-Mamiya R, Noiri E, Isobe H, Nakanishi W, Okamoto K, Doi K, Sugaya T, Izumi T, Homma T, Nakamura E ...see all

Proceedings of the National Academy of Sciences of the United States of America, vol. 107, issue 12 (2010) pp. 5339-44

  • 59

    Readers

    Mendeley users who have this article in their library.
  • 97

    Citations

    Citations of this article.
  • N/A

    Views

    ScienceDirect users who have downloaded this article.
Sign in to save reference

Abstract

Application of nanotechnology to medical biology has brought remarkable success. Water-soluble fullerenes are molecules with great potential for biological use because they can endow unique characteristics of amphipathic property and form a self-assembled structure by chemical modification. Effective gene delivery in vitro with tetra(piperazino)fullerene epoxide (TPFE) and its superiority to Lipofectin have been described in a previous report. For this study, we evaluated the efficacy of in vivo gene delivery by TPFE. Delivery of enhanced green fluorescent protein gene (EGFP) by TPFE on pregnant female ICR mice showed distinct organ selectivity compared with Lipofectin; moreover, higher gene expression by TPFE was found in liver and spleen, but not in the lung. No acute toxicity of TPFE was found for the liver and kidney, although Lipofectin significantly increased liver enzymes and blood urea nitrogen. In fetal tissues, neither TPFE nor Lipofectin induced EGFP gene expression. Delivery of insulin 2 gene to female C57/BL6 mice increased plasma insulin levels and reduced blood glucose concentrations, indicating the potential of TPFE-based gene delivery for clinical application. In conclusion, this study demonstrated effective gene delivery in vivo for the first time using a water-soluble fullerene.

Author-supplied keywords

  • Animals
  • Base Sequence
  • DNA Primers
  • DNA Primers: genetics
  • DNA, Recombinant
  • DNA, Recombinant: administration & dosage
  • DNA, Recombinant: genetics
  • Female
  • Fetus
  • Fetus: metabolism
  • Fullerenes
  • Fullerenes: chemistry
  • Fullerenes: toxicity
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Therapy: methods
  • Green Fluorescent Proteins
  • Green Fluorescent Proteins: genetics
  • Insulin
  • Insulin: genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Nanotechnology
  • Phosphatidylethanolamines
  • Phosphatidylethanolamines: toxicity
  • Pregnancy
  • Recombinant Proteins
  • Recombinant Proteins: genetics
  • Solubility
  • Tissue Distribution
  • Water

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Rui Maeda-Mamiya

  • Eisei Noiri

  • Hiroyuki Isobe

  • Waka Nakanishi

  • Koji Okamoto

  • Kent Doi

Cite this document

Choose a citation style from the tabs below