The in vivo minigene approach to analyze tissue-specific splicing

  • Stoss O
  • Stoilov P
  • Hartmann A
 et al. 
  • 30

    Readers

    Mendeley users who have this article in their library.
  • 46

    Citations

    Citations of this article.

Abstract

The exact mechanisms leading to alternative splice site selection are still poorly understood. However, recently cotransfection studies in eukaryotic cells were successfully used to decipher contributions of RNA elements (cis-factors), their interacting protein components (trans-factors) or the cell type to alternative pre-mRNA splicing. Splice factors often work in a concentration dependent manner, resulting in a gradual change of alternative splicing patterns of a minigene when the amount of a trans-acting protein is increased by cotransfections. Here, we give a detailed description of this technique that allows analysis of large gene fragments (up to 10-12 kb) under in vivo condition. Furthermore, we provide a summary of 44 genes currently investigated to demonstrate the general feasibility of this technique.Themes: Cellular and molecular biologyTopics: Gene structure and function: general Copyright (C) 1999 Elsevier Science B.V.

Author-supplied keywords

  • Alternative splicing
  • Minigene
  • RT-PCR
  • Transfection

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Oliver Stoss

  • Peter Stoilov

  • Annette M. Hartmann

  • Oliver Nayler

  • Stefan Stamm

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free