In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents

  • Zharov V
  • Galanzha E
  • Shashkov E
 et al. 
  • 62

    Readers

    Mendeley users who have this article in their library.
  • 149

    Citations

    Citations of this article.

Abstract

A new photoacoustic flow cytometry was developed for real-time detection of circulating cells, nanoparticles, and contrast agents in vivo. Its capability, integrated with photothermal and optical clearing methods, was demonstrated using a near-infrared tunable laser to characterize the in vivo kinetics of Indocyanine Green alone and single cancer cells labeled with gold nanorods and Indocyanine Green in the vasculature of the mouse ear. In vivo applications are discussed, including selective nanophotothermolysis of metastatic squamous cells, label-free detection of melanoma cells, study of pharmokinetics, and immune response to apoptotic and necrotic cells, with potential translation to humans. The threshold sensitivity is estimated as one cancer cell in the background of 107 normal blood cells.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Vladimir P. Zharov

  • Ekaterina I. Galanzha

  • Evgeny V. Shashkov

  • Nicolai G. Khlebtsov

  • Valery V. Tuchin

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free