In vivo recognition of an RNA aptamer by its transcription factor target

  • Cassiday L
  • Maher L
  • 32

    Readers

    Mendeley users who have this article in their library.
  • 45

    Citations

    Citations of this article.

Abstract

In vitro-selected RNA aptamers are potential inhibitors of disease-related macromolecules. Our laboratory previously isolated an RNA aptamer that specifically binds to the human transcription factor NF-kappaB. This RNA aptamer competitively inhibits DNA binding by NF-kappaB in vitro. In the study presented here, this aptamer was tested for binding to the p50 homodimer form of NF-kappaB (p50(2)) in eukaryotic cells using a yeast three-hybrid system. We show that the alpha-p50 RNA aptamer selectively binds recombinant p50(2) expressed in yeast, demonstrating in vivo recognition of an in vitro-selected RNA aptamer by its protein target. This result suggests that RNA decoys might be used to inhibit the function of DNA-binding proteins in vivo.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • L. A. Cassiday

  • L. J. Maher

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free