Water as a stationary phase modifier in packed-column supercritical fluid chromatography

  • Geiser F
  • Yocklovich S
  • Lurcott S
 et al. 
  • 9


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


A procedure is described for supercritical fluid chromatography (SFC) of free fatty acids (lauric, myristic, palmitic, stearic and arachidic acid) with either dry carbon dioxide or water-saturated carbon dioxide as the mobile phase and with flame-ionization detection. Seven stationary phases were evaluated with water-saturated and dry carbon dioxide under othewise identical conditions. The retention time for lauric acid with water-saturated carbon dioxide varied from 5.6 min (k′ = 8.7) for DeltabondTM Octyl to 23.6 min (k′ = 39.7) for YMC-Gel PVA-SilTM. The range of stationary phase polarity reported in this study should enable an investigator to adjust SFC conditions to resolution requirements for various solute mixtures. Water-saturated carbon dioxide improved the chromatographic resolution and sensitivity of all seven stationary phases, particularly of covalently bonded phases.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • F.O. Geiser

  • S.G. Yocklovich

  • S.M. Lurcott

  • J.W. Guthrie

  • E.J. Levy

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free