Widespread regulation of gene expression in the Drosophila genome by the histone acetyltransferase dTip60

  • Schirling C
  • Heseding C
  • Heise F
 et al. 
  • 18


    Mendeley users who have this article in their library.
  • 5


    Citations of this article.


The MYST histone acetyltransferase (HAT) dTip60 is part of a multimeric protein complex that unites both HAT and chromatin remodeling activities. Here, we sought to gain insight into the biological functions of dTip60. Strong ubiquitous dTip60 knock-down in flies was lethal, whereas knock-down in the wing imaginal disk led to developmental defects in the wing. dTip60 localized to the nucleus in early embryos and was present in a large number of interbands on polytene chromosomes. Genome-wide expression analysis upon depletion of dTip60 in cell culture showed that it regulated a large number of genes in Drosophila, among which those with chromatin-related functions were highly enriched. Surprisingly, a significant portion of these genes were upregulated upon dTip60 loss, indicating that dTip60 has repressive as well as activating functions. dTip60 protein was directly located at promoter regions of a subset of repressed genes, suggesting a direct role in gene repression. Comparison of the gene expression signature of dTip60 downregulation with that of histone deacetylase inhibition with trichostatin A revealed a significant correlation, suggesting that the dTip60 complex recruits an HDAC-containing complex to regulate gene expression in the Drosophila genome.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Corinna Schirling

  • Christiane Heseding

  • Franziska Heise

  • Dörthe Kesper

  • Ansgar Klebes

  • Ludger Klein-Hitpass

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free