Wrestlers’ immune cells produce higher interleukin-6 and lower interleukin-12 and interleukin-13 in response to in vitro mitogen activation

  • Zamani A
  • Omidi M
  • Hemmatfar A
 et al. 
  • 7


    Mendeley users who have this article in their library.
  • 1


    Citations of this article.


Objective(s): Although recent investigations have shown chronic
inflammation and inflammation-associated diseases might be ameliorated
by exercise; little is known about the relation between exercise
training with anti/pro-inflammatory cytokines.
Materials and Methods: This cross sectional study was conducted to
compare interleukin-4 (IL-4), IL-6, IL-10, IL-12, IL-13, interferon
gamma (IFN-gamma) levels in serum, and their in vitro production by
whole blood (WB) cells and by peripheral blood mononuclear cells (PBMCs)
in response to mitogens lipopolysaccharide and phytohemagglutinin.
Twelve elite wrestlers with history of three times per week exercise
training for about 9.5 years, and thirteen healthy silent controls were
recruited. To analysis the cytokines by enzyme linked immunosorbent
assay (ELISA), the blood samples were taken 24 hr after the last
training session from the wrestlers.
Results: Serum analysis for IL-4, IL-6, IL-10, IL-12, IL-13 and
IFN-gamma indicated no statistical difference between the two groups.
Meanwhile, 48 hr in vitro activation of WB and PBMCs by the mitogens
revealed that IL-6 production was elevated in both WB and PBMCs.
Whereas, IL-12 and IL-13 were decreased in supernatant of PBMCs and WB
cells cultures, respectively.
Conclusion: It seems that wrestling cause immune system cells to produce
anti-inflammatory myokine IL-6 and decrease production of
pro-inflammatory cytokine IL-12 and IL-13.

Author-supplied keywords

  • Cytokines
  • Interleukins
  • Mitogens
  • PBMC
  • Whole blood culture
  • Wrestling

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Alireza Zamani

  • Mostafa Omidi

  • Ahmad Hemmatfar

  • Iraj Salehi

  • Hassan Bazmamoun

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free