Antibiotic susceptibility of enterobacteriaceae isolated from costeno artisan cheese sold in monteria department of cordoba, 2009

Abstract

Infections caused by Enterobacteriaceae with multiple resistance to antimicrobial drugs, are a serious problem which must be faced daily by Physician, Veterinarians and Microbiologists. The use of these antibiotics, often indiscriminately, brings with it the selection of resistant mutants. The incidence of different species as well as the level of resistance to antibiotics most commonly used is a phenomenon with its own characteristics so it is essential to monitoring the evolution of resistance in microorganisms. The objective of this study was to determine the susceptibility of Enterobacteriaceae isolated from costeno artisanal cheese, to commonly used antibiotics in the treatment of humans and cattle. Descriptive exploratory study was conducted in 25 retail store in the city of Monteria, capital of the department of Cordoba, Colombia, duly registered with the Secretariat of Health of the department of Cordoba in 2009. 3 samples were taken from store, one every two months for a total of 75 samples, which were evaluated on color, smell and consistency. To isolate and to identify Enterobacteriaceae producing antibiotic resistence, we used the method of the FDA and used TSI, LIA, CITRATE, MR / VP, UREA and SIM. Serology was performed with the Kauffman-White scheme and sensitivity by the agar diffusion method under the international standards issued by the CLSI, 2008. The results were analyzed with the statistical software EXCEL. E. coli were isolated in the 75 samples (100%) tested, 7 of the 75 samples (9,3%) showed Salmonella spp., and in 68 of the 75 samples (90,7%) analyzed was isolated Citrobacter spp. All strains isolated were resistant to amoxycillin for 100%, tetracycline 87.5%, Gentamicin and Chloramphenicol 70% c/u and 62.5% to amikacin. In conclusion, given that the costeno cheese is a food prepared from raw milk, a fact which get worse by deficiencies in sanitation and hygiene of outlets that sell this high regional consumption product, the high resistance percentage of Enterobacteriaceae isolated in these product to antibiotics is a concern, and turn on the alarms on their indiscriminate use, a fact that may cause the emergence of multiresistant strains to transfer this resistance to commensal and to pathogenic bacteria in food and bacteria belonging to the gastrointestinal flora of the consumer, making a serious public health problem.