The turmeric anti-oxidant protein (TAP) had been isolated from the aqueous extract of turmeric. The anti-oxidant principle was found to be a heat stable protein. Trypsin treatment abolished the anti-oxidant activity. The anti-oxidant principle had an absorbance maximum at 280 nm. After gel filtration, the protein showed a 2-fold increase in antioxidant activity and showed 2 bands in the SDS-PAGE with approximate molecular weight range of 24 000 Da. The protein showed a concentration-dependent inhibitory effect on the promoter induced lipid peroxidation. A 50% inhibitory activity of lipid peroxidation was observed at a protein concentration of 50 μg/ml. Ca2+-ATPase of rat brain homogenate was protected to nearly 50% of the initial activity from the lipid peroxidant induced inactivation by this protein. This protection of Ca2+-ATPase activity was found to be associated with the prevention of loss of -SH groups. © 1995.
CITATION STYLE
Selvam, R., Subramanian, L., Gayathri, R., & Angayarkanni, N. (1995). The anti-oxidant activity of turmeric (Curcuma longa). Journal of Ethnopharmacology, 47(2), 59–67. https://doi.org/10.1016/0378-8741(95)01250-H
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