Application of high performance liquid chromatography/evaporative light scattering detector (HPLC/ELSD) for the study and the fractionation of milk fat triacylglycerols

ISSN: 00356808
3Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.

Abstract

The aim of this research was to develop and test a RP-HPLC/ELSD analytical procedure which can be both a suitable method for milk triacylglycerol determination and a useful tool for pre-separation of TAG fractions for further GC/MS identification. This application can be usefully adopted when a Liquid Chromatography/Mass Spectrometry apparatus is not available. RP-HPLC technique, with the conditions chosen, provided, in less than 20 minutes of analysis, a TAG profile including 30 peaks, most of them well resolved. A simple, home-made Stream Splitter System (SSSy) was assembled and used to collect, before the ELSD entrance, both milk fat triacylglycerol fractions and pure triacylglycerol molecules, which were then submitted to GC/MS analysis for identification. Finally the RPHPLC/ELSD technique was applied for the evaluation of the adulteration of milk fat with different amounts (5-50%) of beef tallow and palm kernel oil. The preliminary results suggested that even amounts of the adulterating fats lower than those tested can be detected by the data obtained by RP-HPLC/ELSD profiles.

Cite

CITATION STYLE

APA

Povolo, M., & Contarini, G. (2012). Application of high performance liquid chromatography/evaporative light scattering detector (HPLC/ELSD) for the study and the fractionation of milk fat triacylglycerols. Rivista Italiana Delle Sostanze Grasse, 89(3), 184–192.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free