Comparison of analytical methods for Humic Like Substances ( HULIS ) measurements in atmospheric particles

by C Baduel, D Voisin, J L Jaffrezo
Atmospheric Chemistry and Physics ()


Humic-Like Substances (HULIS) are a major contributor to the organic carbon in atmospheric aerosol. It would be necessary to standardize an analytical method that could be easily and routinely used for HULIS mea- surements. We present one of the first comparisons of two of the main methods in use to extract HULIS, using I) a weak anion exchanger (DEAE) and II) the combination of two separation steps, one according to polarity (on C18) and the second according to acidity (with a strong anion ex- changer SAX). The quantification is performed with a TOC analyzer, complemented by an investigation of the chemi- cal structure of the extracted fractions by UV-Visible spec- troscopy. The analytical performances of each method are determined and compared for humic substances standards. These methods are further applied to determine the water ex- tractable HULIS (HULISWS) and the 0.1M NaOH alkaline extractable HULIS (HULIST) in atmospheric aerosol col- lected in an Alpine Valley during winter time. This compar- ison, although on a limited batch of samples shows that the simpler DEAE isolation procedure leads to higher recoveries and better reproducibility than the C18-SAX procedure, and might therefore be preferable

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