Comparison of DNA polymerases for real-time PCR with SYBR ® Green I

  • Prevost S
  • Bernatchez H
  • Harder C
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Abstract

Nine commercial DNA polymerases were evaluated for real-time PCR performance. The polymerase with the fastest elongation rate was SpeedSTAR™ from Takara. For non-hotstart enzymes, Finnzymes’ Phusion and Biotools’ DNA polymerases had the best balance of speed and minimal primer-dimer formation. For hotstart enzymes, the best performers were SpeedSTAR HS from Takara and KAPA2G Robust from Kapa Biosystems.

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Prevost, S. L., Bernatchez, H., & Harder, C. J. (2008). Comparison of DNA polymerases for real-time PCR with SYBR ® Green I. Spartan Bioscience Inc., 1–3.

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