Generation of pluripotent stem cells from adult human testis

by S Conrad, M Renninger, J Hennenlotter, T Wiesner, L Just, M Bonin, W Aicher, H J Buhring, U Mattheus, A Mack, H J Wagner, S Minger, M Matzkies, M Reppel, J Hescheler, K D Sievert, A Stenzl, T Skutella show all authors
Nature ()
Get full text at journal

Abstract

Human primordial germ cells and mouse neonatal and adult germline stem cells are pluripotent and show similar properties to embryonic stem cells. Here we report the successful establishment of human adult germline stem cells derived from spermatogonial cells of adult human testis. Cellular and molecular characterization of these cells revealed many similarities to human embryonic stem cells, and the germline stem cells produced teratomas after transplantation into immunodeficient mice. The human adult germline stem cells differentiated into various types of somatic cells of all three germ layers when grown under conditions used to induce the differentiation of human embryonic stem cells. We conclude that the generation of human adult germline stem cells from testicular biopsies may provide simple and non-controversial access to individual cell-based therapy without the ethical and immunological problems associated with human embryonic stem cells.

Cite this document (BETA)

Readership Statistics

19 Readers on Mendeley
by Discipline
 
89% Agricultural and Biological Sciences
 
5% Biochemistry, Genetics and Molecular Biology
 
5% Medicine and Dentistry
by Academic Status
 
47% Researcher
 
21% Student > Ph. D. Student
 
16% Professor > Associate Professor
by Country
 
5% Austria

Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Sign up & Download

Already have an account? Sign in