A simple isocratic reversed phase HPLC method for the separation of a series of barbiturates is described. The column used is a newly developed synthetic silica known as Symmetry C18 (Waters Corp., Milford, MA, USA) and the mobile phase consists of 50 mM KH2PO4 (pH 4.55): acetonitrile water (20:30:50:). The barbiturates studied are monitored by UV detection at 225 nm. Detailed methodology and the advantage of the Symmetry C18 reversed phase is presented.
CITATION STYLE
Aboul-Enein, H. Y., Abou-Basha, L. I., & Swartz, M. E. (1997). High performance liquid chromatographic behaviour of several barbiturates on a newly developed high purity silica column. Toxicological and Environmental Chemistry, 58(1–4), 173–177. https://doi.org/10.1080/02772249709358407
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