Huperzine A attenuates the neurotoxic effect of staurosporine in primary rat cortical neurons

by Hai Yan Zhang, Xi Can Tang
Neuroscience Letters ()
Get full text at journal

Abstract

The effects of huperzine A (HupA) on staurosporine-induced neuronal apoptosis and potential mechanisms were investigated in primary cultured rat cortical neurons. Pretreatment of the cells with HupA (0.1-100 μM) 2 h prior to 0.5 μM staurosporine exposure for 24 h, markedly elevated cell survival. Incubation with HupA at higher dose (1 μM) also reduced the DNA fragmentation caused by staurosporine. This dose of HupA also reduced the upregulation of the pro-apoptotic gene, bax, downregulation of the anti-apoptotic gene, bcl-2, and antagonized the decrease in immunoreactive caspase-3 proenzyme. These results demonstrated that HupA protect neurons against staurosporine-induced apoptosis via regulating the apoptotic related genes and caspase-3 proenzyme. © 2003 Elsevier Science Ireland Ltd. All rights reserved.

Cite this document (BETA)

Readership Statistics

11 Readers on Mendeley
by Discipline
 
55% Biological Sciences
 
27% Medicine
 
9% Chemistry
by Academic Status
 
18% Ph.D. Student
 
18% Student (Master)
 
18% Researcher (at a non-Academic Institution)
by Country
 
9% United States

Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Sign up & Download

Already have an account? Sign in