Improved Method of Analysis for Ergovaline in Tall Fescue by High-Performance Liquid Chromatography

Abstract

A rapid HPLC method of analysis for ergovaline in tall fescue plants is presented; it offers the advantages of utilizing readily available materials that are safer to use than those in previous methods. In addition, the sample preparation and Chromatographic method allow for the detection and quantification of both C-8 epimers of the target alkaloid. Inclusion of the epimer in the analysis procedure provides a more accurate method of determining total ergovaline in plant materials.