Isolation and Culture of Soybean (Glycine max L. Merrill) Microspores and Pollen Grains
Protoplasts were isolated from cotton microspores by hydrolysing the\nmicrospore wall with snailase and driselase; the snailase was superior\nto the driselase and the optimum concentration was 0.5%. Sucrose\nwas used as an osmotic regulator, the optimum concentration being\n20%. The protoplasts obtained by this method were cultured in K3\nmedium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre.\nThe first cell divisions were observed after 3-4 days and the second\nafter 10 days. No further cell division was observed, indicating\nthat the culture conditions require further improvement.