Objective: To validate the alkaline hematin technique for measurement of menstrual blood loss using ultra-thin sanitary towels that contain superabsorbent polymer granules as the absorptive agent. Design: Laboratory study using simulated menstrual fluid (SMF) and Always Ultra Normal, Long, and Night "with wings" sanitary towels. Setting: Keele Menstrual Disorders Laboratory. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Recovery of blood, linearity, and interassay variation over a range of SMF volumes applied to towels. Because of the variable percentage of blood in menstrual fluid, blood recovery was assessed from SMF constituted as 10%, 25%, 50%, and 100% blood. The lower limit of reliable detection and the effect of storing soiled towels for up to 4 weeks at 15°C-20°C, 4°C, and -20°C before analysis were determined. Result(s): Ninety percent recovery was reproducibly achieved up to 30 mL applied volume at all tested SMF compositions, except at low volume or high dilution equivalent to <2 mL whole blood. Samples could be stored for 3 weeks at all tested temperatures without loss of recovery. The technique was suitable for processing towels individually or in batches. Conclusion(s): The alkaline hematin technique is a suitable and validated method for measuring menstrual blood loss from Always Ultra sanitary towels that contain superabsorbent polymers. Copyright © 2010 American Society for Reproductive Medicine, Published by Elsevier Inc.
CITATION STYLE
Magnay, J. L., Nevatte, T. M., Dhingra, V., & O’Brien, S. (2010). Menstrual blood loss measurement: Validation of the alkaline hematin technique for feminine hygiene products containing superabsorbent polymers. Fertility and Sterility, 94(7), 2742–2746. https://doi.org/10.1016/j.fertnstert.2010.03.061
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