P-Maleimidophenyl Isocyanate: a Novel Heterobifunctional Linker for Hydroxyl To Thiol Coupling.
- ISSN: 1043-1802
- ISBN: 1043-1802 (Print) 1043-1802 (Linking)
- DOI: 10.1021/bc00021a005
- PubMed: 8324011
p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.