Protein adsorption onto ionic surfaces

by Koichi Kato, Shushi Sano, Yoshito Ikada
Colloids and Surfaces ()


The hydrophobic surface of poly(ethylene terephthalate) fibers was subjected to graft polymerization of non-ionic and ionic monomers in the preparation of non-ionic and ionic hydrophilic polymer surfaces. In addition, ionic hydrogels were prepared by the crosslinking of anionic gelatin in the presence and absence of an anionic polymer. Batch adsorption tests on the grafted surfaces were conducted using proteins with different isoelectric points such as serum albumin, acidic immunoglobulin G, basic immunoglobulin G, and basic fibroblast growth factor. The results of protein adsorption onto these grafted surfaces and protein sorption into the hydrogels could be explained in terms of the electrostatic attraction and repulsion between the charged surfaces and the charged protein molecules. Surfaces grafted with negatively (or positively) charged polymer chains attracted positively (or negatively) charged proteins, whereas grafted surfaces and proteins with the same charge sign exhibited repulsion. Surfaces grafted with non-ionic polymer chains tended to reject protein adsorption, regardless of the isoelectric point of the protein, probably because of steric hindrance by the graft chains.

Cite this document (BETA)

Readership Statistics

6 Readers on Mendeley
by Discipline
by Academic Status
33% Researcher (at an Academic Institution)
17% Researcher (at a non-Academic Institution)
17% Ph.D. Student
by Country
17% Russia
17% France
17% Japan

Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Sign up & Download

Already have an account? Sign in