Memory and logic are central to complex state-dependent computing, and state-dependent behaviors are a feature of natural biological systems. Recently, we created a platform for integrated logic and memory by using synthetic gene circuits, and we demonstrated the implementation of all two-input logic gates with memory in living cells. Here we provide a detailed protocol for the construction of two-input Boolean logic functions with concomitant DNA-based memory. This technology platform allows for straightforward assembly of integrated logic-and-memory circuits that implement desired behaviors within a couple of weeks. It should enable the encoding of advanced computational operations in living cells, including sequential-logic and biological-state machines, for a broad range of applications in biotechnology, basic science and biosensing. © 2014 Nature America, Inc.
CITATION STYLE
Siuti, P., Yazbek, J., & Lu, T. K. (2014). Engineering genetic circuits that compute and remember. Nature Protocols, 9(6), 1292–1300. https://doi.org/10.1038/nprot.2014.089
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