Terminal transferase-dependent polymerase chain reaction (TDPCR) can be used after reverse transcription (RT) to analyze RNA. This method (RT-TDPCR) has been used for study of RNA structure and RNA-protein interactions at nucleotide-level resolution. A detailed protocol of RT-TDPCR is presented here with examples of its use with ribonuclease T1 in mammalian cells for detecting (1) RNA structure and protein footprints of the human ferritin heavy-chain messenger RNA and (2) in vivo structure of exon 4 of human XIST (X chromosome inactiva-tion-specific transcript) RNA. © 2008 Humana Press.
CITATION STYLE
Chen, H. H., LeBon, J., & Riggs, A. D. (2008). Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR. Methods in Molecular Biology, 488, 319–341. https://doi.org/10.1007/978-1-60327-475-3_21
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