Molecular markers play an important role in identifying the species variation, characterizing the genic diversity, and also linking the identified markers to trait of interest. Genome- and transcriptome-derived molecular markers have been widely used to understand the geographical diversity and have also played a major role in the development of high-density linkage maps. In the present protocol, we present a detailed protocol on bioinformatics approaches towards the whole-genome and transcriptome-assisted simple sequence repeats (SSRs) marker mining in Brachypodium distachyon and identification of orthologus SSRs and their validation in Brachypodium ecotypes. We also present a protocol for the validation of the identified markers.
CITATION STYLE
Sablok, G., Mudunuri, S. B., Gudys, K., Chennamsetti, K., Varma, G. P. S., & Kwasniewski, M. (2018). Evaluation of genome-wide markers and orthologous markers in Brachypodium distachyon. In Methods in Molecular Biology (Vol. 1667, pp. 195–201). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7278-4_15
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