High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.
CITATION STYLE
Henneberger, K., Barlow, A., & Paijmans, J. L. A. (2019). Double-stranded library preparation for ancient and other degraded samples. In Methods in Molecular Biology (Vol. 1963, pp. 65–73). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9176-1_8
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