Nuclease activity: an exploitable biomarker in bacterial infections

Abstract

Introduction: In the increasingly challenging field of clinical microbiology, diagnosis is a cornerstone whose accuracy and timing are crucial for the successful management, therapy, and outcome of infectious diseases. Currently employed biomarkers of infectious diseases define the scope and limitations of diagnostic techniques. As such, expanding the biomarker catalog is crucial to address unmet needs and bring about novel diagnostic functionalities and applications. Areas covered: This review describes the extracellular nucleases of 15 relevant bacterial pathogens and discusses the potential use of nuclease activity as a diagnostic biomarker. Articles were searched for in PubMed using the terms: ‘nuclease,’ ‘bacteria,’ ‘nuclease activity’ or ‘biomarker.’ For overview sections, original and review articles between 2000 and 2019 were searched for using the terms: ‘infections,’ ‘diagnosis,’ ‘bacterial,’ ‘burden,’ ‘challenges.’ Informative articles were selected. Expert opinion: Using the catalytic activity of nucleases offers new possibilities compared to established biomarkers. Nucleic acid activatable reporters in combination with different transduction platforms and delivery methods can be used to detect disease-associated nuclease activity patterns in vitro and in vivo for prognostic and diagnostic applications. Even when these patterns are not obvious or of unknown etiology, screening platforms could be used to identify new disease reporters.