Mapping the affinity landscape of Thrombin-binding aptamers on 2F-ANA/DNA chimeric G-Quadruplex microarrays

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Abstract

In situ fabricated nucleic acids microarrays are versatile and very high-throughput platforms for aptamer optimization and discovery, but the chemical space that can be probed against a given target has largely been confined to DNA, while RNA and non-natural nucleic acid microarrays are still an essentially uncharted territory. 2-Fluoroarabinonucleic acid (2F-ANA) is a prime candidate for such use in microarrays. Indeed, 2F-ANA chemistry is readily amenable to photolithographic microarray synthesis and its potential in high affinity aptamers has been recently discovered. We thus synthesized the first microarrays containing 2F-ANA and 2F-ANA/DNA chimeric sequences to fully map the binding affinity landscape of the TBA1 thrombin-binding G-quadruplex aptamer containing all 32 768 possible DNA-to-2F-ANA mutations. The resulting microarray was screened against thrombin to identify a series of promising 2F-ANA-modified aptamer candidates with Kds significantly lower than that of the unmodified control and which were found to adopt highly stable, antiparallel-folded G-quadruplex structures. The solution structure of the TBA1 aptamer modified with 2F-ANA at position T3 shows that fluorine substitution preorganizes the dinucleotide loop into the proper conformation for interaction with thrombin. Overall, our work strengthens the potential of 2F-ANA in aptamer research and further expands non-genomic applications of nucleic acids microarrays.

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Lietard, J., Assi, H. A., Gómez-Pinto, I., González, C., Somoza, M. M., & Damha, M. J. (2017). Mapping the affinity landscape of Thrombin-binding aptamers on 2F-ANA/DNA chimeric G-Quadruplex microarrays. Nucleic Acids Research, 45(4), 1619–1632. https://doi.org/10.1093/nar/gkw1357

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