Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences

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Abstract

Whole-genome sequencing (WGS) has shown immense value in enabling identification and characterization of bacterial taxa. This is particularly true for mycobacteria, where culture-based characterization becomes delayed by the inherently slow growth rate of these organisms. This chapter reviews the general techniques behind WGS and their optimization, existing techniques for species-level identification and the advantages of WGS for this purpose, and a variety of useful tools for the genomic characterization of mycobacterial strains.

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Riojas, M. A., Frank, A. M., Greenfield, S. R., King, S. P., Meehan, C. J., Strong, M., … Hazbón, M. H. (2021). Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences. In Methods in Molecular Biology (Vol. 2314, pp. 399–457). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1460-0_19

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