Understanding of complex protein interactions with respect to anchorage independence

Abstract

Anchorage-independent growth of cells in soft agar is one of the hallmark characteristics of cellular t ransformation and uncontrolled cell growth. It may be considered as one of the most stringent assays for detecting malignant transformation of cells. Here, we describe a retroviral infection of a library of small secretory proteins and the use of the soft agar assay to obtain and study novel interacting protein combinations that cause cell transformation.