RP-HPLC method development and its validation for quantitative determination of rimonabant in human plasma

Abstract

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile:water (90:10, v/v), drug was detected at 260nm using UVdetector. The LOD and LOQ were 3.0 and 10.0g/L, respectively. The method is linear in the interval 50.01000.0g/L. The average extraction recovery of drug from plasma was found to be 92.2. The percent CV of the method was found to be less than 10.8, and accuracy was found between 94.5 and 106.7. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant. Copyright 2012 Shravan Bankey et al.