Comparative In vitro anti-biofilm efficacy of phytosynthesized iron and silver nanoparticles

Abstract

Objective: The objective of the work was to evaluate the efficacy of anti-biofilm activity of green synthesized silver and iron oxide nanoparticles comparatively. Methods: Nanoparticles were synthesized using a rapid, single-step and completely by a green biosynthetic method employing aqueous leaf extracts of Moringa oleifera Lam. The synthesized nanoparticles were characterized by UV-Vis spectrophotometry and X-Ray Diffraction. Bacterial strains used in this study included Staphylococcus epidermidis and Pseudomonas aeruginosa. The biofilm reduction was evaluated through ring test using crystal violet as a staining agent. Results: Colour change was observed after half an hour, which indicated the formation of silver and iron nanoparticles. Synthesis of nanoparticles was confirmed by UV and XRD. The anti-biofilm forming ability of AgNPs and FeNPs were compared with standard antibiotic. It was found that FeNPs showed more biofilm destruction ability (58%) for S. compared to P. aeruginosa (50%) and standard (30%). Whereas, AgNPs displayed significant biofilm destruction ability (78%) for P. aeruginosa compared to S. epidermidis (43%) and standard (34%). Conclusion: Based on the results obtained in this investigation, it is concluded that FeNPs have anti-biofilm activity against S. epidermidis whereas AgNPs have anti-biofilm activity against P. aeruginosa.