In vitro mass propagation of sugarcane (Saccharum officinarum L.) var. Isd 32 through shoot tips and folded leaves culture

Abstract

Plant tissue culture is the most commercially successful aspect of plant biotechnology, which has introduced an exciting new phase into plant propagation and breeding. This paper presents a research finding that establish an efficient regeneration method for mass propagation of sugarcane var. Isd 32 using shoot tip and folded leaf as expiants. In this study, in vitro shoot tip and folded leaf culture techniques were used for mass propagation and quality production of sugarcane plantlets. The expiants of sugarcane var. Isd 32 was cultured on MS + 1.5 mg L-1 BA + 0.5 mg L-1 NAA for shoot tip and MS + 0.5 mg L-1 Kin + 5.0 mg L-1 NAA for folded leaf. Culture after 30 days, about 90 and 80% of the shoot tip and folded leaf explants, respectively were successfully regenerated shoots. The average number and length of shoots from shoot tip expiants was observed as 17.20±1.30 and 7.20±1.40 cm, respectively. Similarly, the average number and length of shoots from folded leaf explants was observed as 20.20±2.20 and 6.70±1.20 cm, respectively. Presence of callus and morphological variants were not observed during the passage of in vitro culture. There is no efficient regeneration technique that had been established for mass propagation of sugarcane var. Isd 32. The proposed technique can be used to enhance mass production of sugarcane crop economically especially with the present trend of demand of sugarcane in the region. © 2007 Asian Network for Scientific Information.