Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. Results: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed. Conclusion: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.
CITATION STYLE
Kim, D. E., Lee, J. H., Ji, K. B., Park, K. S., Kil, T. Y., Koo, O., & Kim, M. K. (2022). Generation of genome-edited dogs by somatic cell nuclear transfer. BMC Biotechnology, 22(1). https://doi.org/10.1186/s12896-022-00749-3
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