Crosslinked hemoglobin-superoxide dismutase-catalase scavenges free radicals in a rat model of intestinal ischemia-reperfusion injury

Abstract

A rat model of isolated intestinal ischemia-perfusion was developed to compare the effects of crosslinked hemoglobin (PolyHb) versus crosslinked hemoglobin-superoxide dismutase-catalase (PolyHb-SOD-CAT) on free radical in ischemia-reperfusion. The rats underwent midline laparotomy and ligation. The system was flushed of blood and the portal vein was cannulated. Perfusates containing modified hemoglobin and 4-hydroxybenzoate (4 HB) were infused. Hydroxyl generation was assessed by an aromatic hydroxylation technique. The levels of 3,4 dihydroxybenzoate were compared. It was indicated that PolyHb-SOD-CAT is effective in reducing hydroxyl radical generation following reperfusion and a possible protective perfusate in intestinal reperfusion injury.