Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer's electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Shiva, S., Vu, H. S., Roth, M. R., Zhou, Z., Marepally, S. R., Nune, D. S., … Welti, R. (2013). Lipidomic analysis of plant membrane lipids by direct infusion tandem mass spectrometry. Methods in Molecular Biology, 1009, 79–91. https://doi.org/10.1007/978-1-62703-401-2_9
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