Efficient propagation of measles virus in suspension cultures

Abstract

Suspension cultures of a human prostate cell line (MA160) supported abundant growth of the Edmonston strain of measles virus. The virus yields obtained with these suspension cultures (150 to 800 PFU/cell) were at least 20- to 100-fold higher than those frequently reported in the literature. Monolayer cultures of MA160 cells did not support a virus replication nearly as efficiently (progeny yield, 25 PFU/cell).