Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosis

Abstract

BackgroundCytotoxic T lymphocytes take on a leading role in many immune-related diseases. They function as key effector immune cells fighting cancer cells, but they are also considerably involved in autoimmune diseases. Common to both situations, CD8(+) T cells need to adapt their metabolism and effector function to the harsh and nutrient-deprived conditions of the disease-associated microenvironment. MethodsWe used an in vitro starvation as well as rapamycin treatment protocol mimicking nutrient deprivation to generate CD8(Low) versus CD8(High) T cells and performed FACS-Sorting followed by transcriptomic profiling of the cytotoxic T cell subsets. Prominent markers identified in the CD8(Low) versus the CD8(High) T cells were then used to investigate the presence of these cell subsets in immune-related human diseases. Employing cancer tissue microarrays and PhenOptics multispectral imaging as well as flow cytometry, we studied these CD8(+) T cell subsets in cancer and relapsing-remitting multiple sclerosis patients. ResultsStarvation induced a decreased expression of CD8, yielding a CD8(Low) T cell subpopulation with an altered transcriptomic signature and reduced effector function. CD8(Low) T cell showed enhanced ST2L and IL6ST (CD130) expression compared to CD8(High) T cells which expressed elevated KLRD1 (CD94) and granzyme B levels within the tumour microenvironment (TME). Spatial analysis revealed the presence of CD8(High) T cells in close proximity to tumour cells, while the CD8(Low) T cells resided at the tumour boundaries. Importantly, the number of tumour-infiltrating CD8(Low) T lymphocytes correlated with a poor prognosis as well as with enhanced cancer progression in human mammary carcinoma. We also found a reduced frequency of CD8(Low) T lymphocytes in a cohort of relapse (disease active) multiple sclerosis patients compared to healthy subjects during immune cell starvation in vitro. ConclusionsIn summary, our data show that functionally distinct cytotoxic T lymphocytes can be identified based on their expression of CD8. Indicating a more general role in CD8 T cell immunity, these cells may play opposing roles in the TME, and also in the pathophysiology of autoimmune diseases such as multiple sclerosis.