Plastid-associated galactolipid composition in eyespot-containing dinoflagellates: A review

Abstract

Relative to the large number of photosynthetic dinoflagellate species, only a select few possess proteinaceous, carot-enoid-rich eyespots which have been demonstrated in other algae to act in phototactic responses. The proteins com-prising the different categories of dinoflagellate eyespots are positioned in or near the peridinin-containing photosynthetic plastid membranes which are composed primarily of two galactolipids, mono-and digalactosyldiacylglycerol (MGDG and DGDG). Within eyespot-containing dinoflagellates, this arrangement occurs mostly in those with secondary plastids, although some dinoflagellates with tertiary plastids of diatom origin are known to possess eyespots. We here provide an examination of the MGDG and DGDG composition of eyespot-containing dinoflagellates with secondary, peridinin-containing plastids and tertiary plastids of diatom origin to address the fundamental question of whether eyespots and their component proteins and carotenoids are associated with alterations in galactolipid composition when compared to eyespot-lacking photosynthetic dinoflagellates. This is an important question because the dinofla-gellate eyespot-plastid membrane system can be considered a more complicated and evolved state of plastid develop-ment. Included in this examination are data on the previously unexamined peridinin-and type A eyespot-containing dinoflagellate Margalefidinium polykrikoides, and the type D eyespot-containing, aberrant plastid “dinotom” Durinskia baltica. In addition, we have reviewed the galactolipid composition of algae from the Chlorophyceae, Cryptophyceae, and Euglenophyceae as a comparison to determine if algal classes apart from the Dinophyceae contain altered galacto-lipids in association with eyespots. We conclude that the presence of an eyespot in dinoflagellates and other algae is not associated with noticeable changes in galactolipid composition.