Nanopore technology enables the detection and analysis of single protein molecules. The technique measures the ionic current passing through a single pore inserted in an electrically insulating membrane. The translocation of the protein molecule through the pore causes a modulation of the ionic current. Analysis of the ionic current reveals the biophysics of co-translocational unfolding and may be used to infer the amino acid sequence and posttranslational modifications of the molecule.
CITATION STYLE
Celaya, G., & Rodriguez-Larrea, D. (2021). Oligonucleotide-directed protein threading through a rigid nanopore. In Methods in Molecular Biology (Vol. 2186, pp. 135–144). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0806-7_10
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