Development and Evaluation of Natural Hydroxyapatite Ceramics Produced by the Heat Treatment of Pig Bones

Abstract

by the Heat Treatment of Pig Bones Ki-Taek Lim, Jin-Woo Kim, Jangho Kim, Jong Hoon Chung* Department of Biosystems \& Biomaterials Science and Engineering, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea Department of Biological and Agricultural Engineering, Institute for Nanoscience \& Engineering, University of Arkansas, Fayetteville, AR 72701, United States Received: June 26th 2014; Revised: July 14th 2014; Accepted: August 20th 2014 Purpose: The aim of this research was to develop and evaluate natural hydroxyapatite (HA) ceramics produced from the heat treatment of pig bones. Methods: The properties of natural HA ceramics produced from pig bones were assessed in two parts. Firstly, the raw materials were characterized. A temperature of 1,200°C was chosen as the calcination temperature. Fine bone powders (BPs) were produced via calcinations and a milling process. Sintered BPs were then characterized using field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared (FTIR) spectroscopy, and a 2-year in vitro degradability test. Secondly, an indirect cytotoxicity test was conducted on human osteoblast-like cells, MG63, treated with the BPs. Results: The average particle size of the BPs was 20 ± 5 μm. FE-SEM showed a non-uniform distribution of the particle size. The phase obtained from XRD analysis confirmed the structure of HA. Elemental analysis using XRF detected phosphorus (P) and calcium (Ca) with the Ca/P ratio of 1.6. Functional groups examined by FTIR detected phosphate (PO43-), hydroxyl (OH-), and carbonate (CO32-). The EDX, XRF, and FTIR analysis of BPs indicated the absence of organic compounds, which were completely removed after annealing at 1,200°C. The BPs were mostly stable in a simulated body fluid (SBF) solution for 2 years. An indirect cytotoxicity test on natural HA ceramics showed no threat to the cells. Conclusions: In conclusion, the sintering temperature of 1,200°C affected the microstructure, phase, and biological characteristics of natural HA ceramics consisting of calcium phosphate. The Ca–P-based natural ceramics are bioactive materials with good biocompatibility; our results indicate that the prepared HA ceramics have great potential for agricultural and biological applications.