RNA-protein interactions govern every aspect of RNA metabolism, and aberrant RNA-binding proteins are the cause of hundreds of genetic diseases. Quantitative measurements of these interactions are necessary in order to understand mechanisms leading to diseases and to develop effi cient therapies. Existing methods of RNA-protein interactome capture can afford a comprehensive snapshot of RNA-protein interaction networks but lack the ability to characterize the dynamics of these interactions. As all ensemble methods, their resolution is also limited by statistical averaging. Here we discuss recent advances in single molecule techniques that have the potential to tackle these challenges. We also provide a thorough overview of single molecule colocalization microscopy and the essential protein and RNA tagging and detection techniques.
CITATION STYLE
Serebrov, V., & Moore, M. J. (2016). Single molecule approaches in RNA-protein interactions. In Advances in Experimental Medicine and Biology (Vol. 907, pp. 89–106). Springer New York LLC. https://doi.org/10.1007/978-3-319-29073-7_4
Mendeley helps you to discover research relevant for your work.